J Korean Med Sci.  2008 Jun;23(3):365-372. 10.3346/jkms.2008.23.3.365.

Impact of Glutathione S-Transferase M1 and T1 Gene Polymorphisms on the Smoking-Related Coronary Artery Disease

• Kim SJ
• Kim MG
• Kim KS
• Song JS
• Yim SV
• Chung JH

• KMID: 1786870
• DOI: http://doi.org/10.3346/jkms.2008.23.3.365

Abstract

Glutathione S-transferase (GST) plays a key role in the detoxification of xenobiotic atherogen generated by smoking. To analyze the effect of GSTM1/T1 gene polymorphisms on the development of smoking-related coronary artery disease (CAD), 775 Korean patients who underwent coronary angiography were enrolled. The subjects were classified by luminal diameter stenosis into group A (>50%), B (20-50%), or C (<20%). GSTM1 and GSTT1 gene polymorphisms were analyzed using multiplex polymerase chain reaction (PCR) for GSTM1/T1 genes and CYP1A1 gene for internal control. Of 775 subjects, 403 patients belonged to group A. They had higher risk factors for CAD than group B (N=260) and group C (N=112). The genotype frequencies of null GSTM1 and GSTT1 showed no significant differences among 3 groups. Considering the effect of GSTM1 gene polymorphisms on the smoking-related CAD, smokers with GSTM1 null genotype had more increased risk for CAD than non-smoker with GSTM1 positive genotype (odds ratios [OR], 2.07, confidence interval [CI], 1.06-4.07). Also the effect of GSTT1 gene polymorphism on smoking-related CAD showed the same tendency as GSTM1 gene (OR, 2.00, CI, 1.05-3.84). This effect of GSTM1/T1 null genotype on smoking-related CAD was augmented when both gene polymorphisms were considered simultaneously (OR, 2.76, CI, 1.17-6.52). We concluded that GSTM1/T1 null genotype contributed to the pathogenesis of smoking-related CAD to some degree.

Keyword

Glutathione Transferase; Polymorphism, Genetic; Smoking; Coronary Artery Disease

MeSH Terms

Aged
Coronary Angiography
Coronary Artery Disease/epidemiology/*genetics/radiography
Female
Genetic Predisposition to Disease/epidemiology
Genotype
Glutathione Transferase/*genetics
Humans
Male
Middle Aged
*Polymorphism, Genetic
Risk Factors
Severity of Illness Index
Smoking/epidemiology/*genetics

Figure

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  Fig. 1 Polymerase chain reaction results of GSTM1/T1 genes. GST, glutathione S-transferase.

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