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Single step purification of potent antigenic protein from sparganum by gelatin-affinity chromatography

Kong Y, Kang SY, Cho SY

Out of many component proteins in crude saline extract of Spirometra mansoni plerocercoid (sparganum), 36 kDa and 29 kDa proteins were found to be the most antigenic and were already...
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Antigen specificity of 36 and 31 kDa proteins of Spirometra erinacei plerocercoid in tissue invading nematodiasis

Morakote N, Kong Y

Diagnostic specificity of 36 and 31 kDa proteins of Spirometra erinacei plerocercoid (sparganum) was evaluated by micro-ELISA in tissue invading nematodiasis such as 25 gnathostomiasis, 33 angiostrongyliasis, 22 trichinellosis patients,...
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Impact of helminth infection on childhood allergic diseases in an area in transition from high to low infection burden

Amarasekera M, Gunawardena NK, de Silva N, Douglass J, O'Hehir RE, Weerasinghe A

BACKGROUND: The effect of helminth infections on allergic diseases is still inconclusive. Furthermore, the effect of helminth infections on childhood allergic diseases in a tropical area where prevalence of helminth...
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Double Strand Problems: Reverse DNA Sequences Deposited in the DNA Database

Thaenkham , Nawa Y

No abstract available.
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Purification of antigenic protein of sparganum by immunoaffinity chromatography using a monoclonal antibody

Cho SY, Kang SY, Kong Y

The quality improvement of antigen (crude saline extract) of Spirometra mansoni pleroceroid (sparganum) was investigated by protein purification. The crude extract was fractionated by gel filtration through Sephacryl S-300 Superfine....
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Tissue origin of soluble component proteins in saline extract of adult Paragonimus westermani

Kong Y, Park CY, Kang SY, Cho SY

Tissue origin of individual component proteins in crude extract of adult Paragonimus westermani was investigated. Major soluble component proteins were separated by disc-PAGE in 8% slab gel. By predefined Rf...
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Immunohistochemical localization of 36 and 29 kDa proteins in sparganum

Kim LS, Kong Y, Kang SY, Cho SY

Antigenic proteins of 36 and 29 kDa were localized in Spirometra mansoni plerocercoid (sparganum) immunohistochemically by avidin biotin complex (ABC) staining. When polyclonal antibodies such as BALB/c mouse serum immunized...
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A Parasitic Infection with Eosinophilia in a University Medical Center

Ryu JH, Jho HJ, Chang YS, Cho BL, Huh BY

  • KMID: 2294581
  • J Korean Acad Fam Med.
  • 2006 Jul;27(7):529-533.
BACKGROUND: The causes of eosinophilia are allergies, parasite infections, medications, skin diseases, and others. But, the etiologies of eosinophilia and variables associated with eosinophilia are not well known in Korea. METHODS:...
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An evaluation of cellophane thick smear technique for mass stool examination

Cho SY, Lee SH, Rim HJ, Seo BS

To evaluate the Kato's cellophane thick smear techcique in detection ability of various helminth ova, authors examined 1,843 nationwidely collected stool specimens by the two methods, formalin-ether technique and cellophane...
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Protein composition and antigenicity of the tegument from Paragonimus westermani

Kim SI, Cho SY

To ascertain that tegument of Paragonimus westermani has specific antigenic proteins, the tegumental fraction was isolated from 10-month-old worms by 0.1% digitonin solution, and subjected to SDS-PAGE and immunoblot. Component...
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Eosinophil and Tissue-invasive Parasitic Helminth

Shin MH

Eosinophils are primarily tissue resident cells, and play important roles in host's immune responses and maintenance of chronic infection during infection with tissue-invasive parasitic helminth. Such parasite secretes particular molecules...
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Recent demonstration of helminth eggs and larvae from vegetable cultivating soil

Choi DW, Ock MS, Suh JW

A survey on soil samples collected from the vegetable gardens and vinyl houses in the vicinity of Taegu was conducted for the discovery of helminth eggs and larvae from October...
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Immunohistochemical observation on the antigens inducing IgG and IgM antibodies against sparganum

Kim CH, Choi WS

Localization and characterization of the antigenic components of sparganum which induced IgG and IgM antibodies in the host were studied by immunohistochemical techniques and SDS-PAGE and Western blotting. The antigen...
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Eosinophil-Mediated Tissue Inflammatory Responses in Helminth Infection

Shin MH, Lee YA, Min DY

Eosinophilic leukocytes function in host protection against parasitic worms. In turn, helminthic parasites harbor specific molecules to evade or paralyze eosinophil-associated host immune responses; these molecules facilitate the migration and...
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Immunological Mechanisms by Which Concomitant Helminth Infections Predispose to the Development of Human Tuberculosis

Mendez-Samperio P

  • KMID: 1792119
  • Korean J Parasitol.
  • 2012 Dec;50(4):281-286.
Helminthic infections afflict over 1.5 billion people worldwide, while Mycobacterium tuberculosis infects one third of the world's population, resulting in 2 million deaths per year. Although tuberculosis and helminthic infections...
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Metagonimus yokogawai: a 100-kDa Somatic Antigen Commonly Reacting with Other Trematodes

Han ET, Yang HJ, Park YJ, Park JH, Chai JY

  • KMID: 1792170
  • Korean J Parasitol.
  • 2014 Apr;52(2):201-204.
This study was undertaken to characterize the properties of a 100 kDa somatic antigen from Metagonimus yokogawai. Monoclonal antibodies (mAbs) were produced against this 100 kDa antigen, and their immunoreactivity...
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Recent patterns of intestinal helminth infections among the residents in Taegu City, Korea

Joo CY

In order to estimate the recent pattens of intestinal helminth infections among the residents in Taege City, Korea, a survey based on discovery of helminth eggs by formalin-ether sedimentation and...
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Genetic comparison between Spirometra erinacei and S. mansonoides using PCR-RFLP analysis

Lee SU, Huh S, Phares CK

The only observed morphological difference between Spirometra erinacei and S. mansonoides is the uterine shape of the mature proglottid. Two species of worms are thought to be evolutionarily closely...
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Immune reactions between excretory-secretory antigens and specific antibodies of Clonorchis sinensis before and after praziquantel treatment in experimentally infected rabbits

Kim SI

This study was designed to evaluate the humoral immune reactions in clonorchiasis before and after praziquantel treatment. Rabbits were infected with 150 or 450 metacercariae, treated on 4 and 8...
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Autoradiographic studies on some parasitic helminth

Yoon JS, Lee SH, Seo BS

Direct contact method of autoradiography was utilized in studying the distribution of exogenous C(14)-proline in Clonorchis sinensis, Eurytrema pancreaticum, Hymenolepis diminuta and Dipylidium caninum. The most distinct autoradiogram were obtained...
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