- Characterization of Insect Cells Transformed with Autographa calfornica Nuclear Polyhedrosis Virus IE1 Gene
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Cho ES, Park HJ, Lee KS, Kang SW, Yun EY, Kim KY, Sohn HD, Jin BR
- KMID: 2096748
- J Korean Soc Virol.
- 1999 Jun;29(2):137-144.
Transformation efficiency, virus multiplication and foreign gene expression were characterized in the insect cells transformed with Autographa calfornica nuclear polyhedrosis virus (AcNPV) immediate early 1 gene (IE1). Transformation efficiency of... -
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- Expression of Green Fluorescent Protein in Both Spodoptera frugiperda Cells and Bombyx mori Larvae by Ac-Bm Hybrid Virus
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Jin BR, Yoon HJ, Yun EY, Kang SW, Cho ES, Kang SK
- KMID: 1551989
- J Korean Soc Virol.
- 1998 Sep;28(3):225-232.
We have expressed GFP in Sf9 and Bm5 cells or Bombyx by larvae by using Ac-Bm hybrid virus capable of replicating in both Bm5 and Sf9 cells. Genomic DNA of... -
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- Expression of Phospholipase C-B3 using Recombinant Baculovirus Expression System
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Park DJ
- KMID: 2331069
- J Korean Soc Endocrinol.
- 1997 Jun;12(2):283-294.
BACKGROUND: Although phospholipase C (PLC)-B3 is thought to be a very important enzyme in intracellular signal transduction, the sophisticated and complicated purification steps make it difficult to obtain sufficient amount... -
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- Expression of Human Papillomavirus Type 16, Prototype and Natural Variant E7 Proteins using Baculovirus Expression System
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Han HS, Kee SH, Kim HJ, Cho KA, Kim YW, Cho MK, Chang WH, Hwang SB
- KMID: 1940945
- J Korean Soc Virol.
- 1998 Mar;28(1):53-62.
Human papillomavirus (HPV) 16, E7 proteins derived from the prototype (Bac73) and natural variant (Bac101) E7 open reading frame were produced in Sf9 insect cells. The variant E7 gene occurred... -
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- Codon optimization of the rabbit hemorrhagic disease virus (RHDV) capsid gene leads to increased gene expression in Spodoptera frugiperda 9 (Sf9) cells
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Gao J, Meng C, Chen Z, Li C, Liu G
- KMID: 1712311
- J Vet Sci.
- 2013 Dec;14(4):441-447.
- doi: 10.4142/jvs.2013.14.4.441
Rabbit hemorrhagic disease (RHD) is contagious and highly lethal. Commercial vaccines against RHD are produced from the livers of experimentally infected rabbits. Although several groups have reported that recombinant subunit... -
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- Sequence Analysis and Expression of the VP7 Gene of G1 Rotavirus Isolated from an Infant in Korean
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Song MO, Park CM, In SJ, Kim KJ, Chung SI, Choi CS, Kim WY, Lim IS
- KMID: 1551993
- J Korean Soc Virol.
- 1998 Sep;28(3):247-265.
To determine the sequence and expression of the VP7 gene of Korean isolates (CAU-9), viral RNA was purified and used for cDNA amplification by RT-PCR. The VP7 cDNA was cloned,... -
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- Mouse Dual Ig Domain Containing Cell Adhesion Molecule Protein Expression and Purification Using the Baculovirus Expression Vector System
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Park SW, Choi JH, Goo TW, Kim SR, Lee GG, Kang SW
- KMID: 2055049
- J Bacteriol Virol.
- 2010 Sep;40(3):123-130.
- doi: 10.4167/jbv.2010.40.3.123
A baculovirus expression vector system (BEVS) is used routinely to produce recombinant proteins in the milligram scale. Dual Ig domain containing cell adhesion molecule (DICAM) belongs to the type I... -
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- Sequence Analysis and Expression of ORF5 in Korean Isolate of Porcine Reproductive and Respiratory Syndrome Virus
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Yi SS, Sun JS, Kim HS, Kang SY, Hahn TW
- KMID: 1449892
- J Bacteriol Virol.
- 2011 Sep;41(3):173-181.
- doi: 10.4167/jbv.2011.41.3.173
Porcine reproductive and respiratory syndrome virus (PRRSV) is the causative agent of reproductive failure and respiratory disorders in pigs. The viral genome consists of eight overlapping open reading frames (ORFs).... -
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- Protein Expression of the Bombyx mori Decapentaplegic Gene using the Baculovirus Expression Vector System
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Jeong ST, Park SW
- KMID: 2068741
- J Bacteriol Virol.
- 2015 Sep;45(3):256-261.
- doi: 10.4167/jbv.2015.45.3.256
The Bombyx mori decapentaplegic gene is one of the conserved genes in vertebrate and invertebrates. The TGF-beta superfamily contains conserved polypeptide growth factors that play important roles in different cellular... -
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- Activation of DDR2 Involved in Atherosclerosis by Oxidative Stress
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Yang K, Yang BS
- KMID: 2015171
- J Korean Geriatr Soc.
- 2007 Dec;11(4):181-188.
BACKGROUND: Reactive Oxygen species have been known to be a key factor to promote atherosclerosis. DDR2(Discoidin Domain Receptor 2) is a cell surface receptor tyrosine kinase which is activated... -
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- Expression and Purification of Phospholipase C-beta4, and Chimeric Phospholipase C and Characterization of Them
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Park DJ
- KMID: 1973790
- Endocrinol Metab.
- 2012 Dec;27(4):282-288.
- doi: 10.3803/EnM.2012.27.4.282
BACKGROUND: Phospholipase C-beta4 (PLC-beta4) is known to be one of the most important signal transducing molecules; however, its biophysical and chemical characteristics are not well known due to the difficulty... -
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- Evaluation of JEV and BVDV Clearance During the Purification of Recombinant HPV-16 L1 Virus-Like Particles
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Choi JY, Jeong HS, Kim YL, Son HK, Kim BG, Ryu SR, Shin JH, Baek SY, Min HK, Park SN
- KMID: 1963269
- J Bacteriol Virol.
- 2004 Sep;34(3):213-221.
Insect cell-derived biotechnological products have a potential for viral contamination from cell line sources themselves or from adventitious introduction of virus during production. The objective of this study was to... -
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- A Quantitative Assay of Japanese Encephalitis Virus as a Model Virus for Viral Clearance Validation in Insect Cell-Derived Biotechnology Products
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Jeong HS, Park YN, Choi JY, Kim YL, Kim BG, Ryu SR, Shin JH, Baek SY, Lee SH, Park SN
- KMID: 1573028
- J Bacteriol Virol.
- 2002 Jun;32(2):187-194.
Commercial interests towards insect cell cultures have greatly been increased recently, in part due to the widespread use of insect virus-based vectors for efficient expressions of foreign proteins. Insect cell-derived... -
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- Development of a Rapid Diagnostic Test Kit to Detect IgG/IgM Antibody against Zika Virus Using Monoclonal Antibodies to the Envelope and Non-structural Protein 1 of the Virus
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Kim YH, Lee J, Kim YE, Chong CK, Pinchemel , Reisdörfer F, Coelho JB, Dias RF, Bae PK, Gusmão ZP, Ahn HJ, Nam HW
- KMID: 2408526
- Korean J Parasitol.
- 2018 Feb;56(1):61-70.
- doi: 10.3347/kjp.2018.56.1.61
We developed a Rapid Diagnostic Test (RDT) kit for detecting IgG/IgM antibodies against Zika virus (ZIKV) using monoclonal antibodies to the envelope (E) and non-structural protein 1 (NS1) of ZIKV.... -
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- Preparation and diagnostic utility of a hemagglutination inhibition test antigen derived from the baculovirus-expressed hemagglutinin-neuraminidase protein gene of Newcastle disease virus
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Choi KS, Kye SJ, Jeon WJ, Park MJ, Kim S, Seul HJ, Kwon JH
- KMID: 1705559
- J Vet Sci.
- 2013 Sep;14(3):291-297.
- doi: 10.4142/jvs.2013.14.3.291
A recombinant hemagglutinin-neuraminidase (rHN) protein from Newcastle disease virus (NDV) with hemagglutination (HA) activity was expressed in Spodoptera frugiperda cells using a baculovirus expression system. The rHN protein extracted from... -
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