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Cloning and sequencing of p33 in a Korean isolate of Theileria sergenti

Kang SW, Choi EJ, Kweon CH

The gene encoding the 33 kDa piroplasm surface protein of Theileria sergenti isolated in Korea was cloned and the nucleotide sequence was determined by dideoxy chain termination method. The cloned...
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Studies of cocktail therapy with multiple cytokines for neoplasia or infectious disease of the dog I. cDNA cloning of canine IL-3 and IL-6

Shin IS, Kim HR, Nam MJ, Youn HY

  • KMID: 1103518
  • J Vet Sci.
  • 2001 Aug;2(2):115-120.
This paper describes the cloning and sequence analysis of the cDNAs encoding the canine homologues of interleukin-3 (IL-3) and interleukin-6 (IL-6). The coding sequences for canine IL-3 and IL-6 were...
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Genetic Variation in the NSP4 Gene of Human Rotavirus Isolated in Seoul

Cho SL, Ahn JH, Kim K, Chung SI, Lim I, Kim W

The nonstructural protein 4 (NSP4) of rotavirus encoded by gene 10, plays an important role in rotavirus pathogenicity. In this study, NSP4 gene sequences of human rotaviruses circulating in Seoul,...
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Sequence Analysis and Expression of the VP7 Gene of G1 Rotavirus Isolated from an Infant in Korean

Song MO, Park CM, In SJ, Kim KJ, Chung SI, Choi CS, Kim WY, Lim IS

  • KMID: 1551993
  • J Korean Soc Virol.
  • 1998 Sep;28(3):247-265.
To determine the sequence and expression of the VP7 gene of Korean isolates (CAU-9), viral RNA was purified and used for cDNA amplification by RT-PCR. The VP7 cDNA was cloned,...
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Purification and cloning of glyoxalase II from rat liver

Cho MY, Bae CD, Park JB, Lee TH

  • KMID: 754988
  • Exp Mol Med.
  • 1998 Mar;30(1):53-57.
Glyoxalase (GLO) II, which is a component of GLO system and catalyze the conversion of S-lactoyl-glutathione to D-lactate, was purified 1488 fold from rat liver by two steps of Affigel blue and...
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Differential diagnosis of Salmonella gallinarum and S. pullorum using PCR-RELP

Park MK, Choi KS, Kim MC, Chae JS

  • KMID: 1119218
  • J Vet Sci.
  • 2001 Dec;2(3):213-219.
Salmonellosis in poultry of Korea is a significant health problem, which causes substantial economic losses. The most common causative agents of chicken salmonellosis ar S. gallinarum and S. pullorum. Traditional...
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Molecular cloning of clathrin assembly protein gene (rCALM) and its differential expression to AP180 in rat brain

Kim HL, Lee SC

  • KMID: 1029926
  • Exp Mol Med.
  • 1999 Dec;31(4):191-196.
Binding of clathrin assembly protein to clathrin triskelia induces their assembly into clathrin-coated vesicle (CCV) in neurons. The clathrin assembly protein gene (rCALM) was cloned from rat brain...
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Detection and molecular characterization of infectious bronchitis virus isolated from recent outbreaks in broiler flocks in Thailand

Pohuang T, Chansiripornchai N, Tawatsin A, Sasipreeyajan J

Thirteen field isolates of infectious bronchitis virus (IBV) were isolated from broiler flocks in Thailand between January and June 2008. The 878-bp of the S1 gene covering a hypervariable region...
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Cloning and expression of trypsin-encoding cDNA from Blattella germanica and its possibility as an allergen

Ock MS, Kim BJ, Kim SM, Byun KH

In this study, the trypsin gene (bgtryp-1) from the German cockroach, Blattella germanica, was cloned via the immunoscreening of patients with allergies to cockroaches. Nucleotide sequence analysis predicted an 863...
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Differentially expressed genes of Acanthamoeba castellanii during encystation

Moon EK, Chung DI, Hong YC, Kong HH

To examine the expressed gene profile during encystation of Acanthamoeba castellanii Castellani, we used differentially expressed gene (DGE) screening by RT-PCR with 20 sets of random primers. From this analysis,...
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Cloning of canine GM-CSF and SCF genes

Shin IS, Nam MJ, Park SJ, Youn HY, Han HR

  • KMID: 1119211
  • J Vet Sci.
  • 2001 Dec;2(3):159-166.
Cytokines have pleiotropic regulatory effects on hematopoietic cells and many other cell types that participate in host defence and repair processes. Granulocyte-macrophage colony-stimulating factor (GM-CSF) mediates the growth and differentiation...
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Sequence analysis of the S1 glycoprotein gene of infectious bronchitis viruses: identification of a novel phylogenetic group in Korea

Jang JH, Sung HW, Song CS, Kwon HM

Twelve Korean infectious bronchitis viruses (IBVs) were isolated in the field from chickens suspected of being carriers of infectious bronchitis between 2001 and 2003. The S1 glycoprotein genes of these...
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Isolation and identification of a canine coronavirus strain from giant pandas (Ailuropoda melanoleuca)

Gao FS, Hu GX, Xia XZ, Gao YW, Bai YD, Zou XH

Two giant pandas (Ailuropoda melanoleuca) died of unknown causes in a Chinese zoo. The clinical disease profile suggested that the pandas may have suffered a viral infection. Therefore, a series...
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The new genotypic human calicivirus isolated in Seoul

Han DP, Lee HW, Sohn JH, Yeh BI, Choi JW, Kim HW

  • KMID: 927127
  • Exp Mol Med.
  • 2000 Mar;32(1):6-11.
A new type of human calicivirus (HuCV) showing the classic cup-shaped surface morphology was identified in the stool sample from a child with symptoms of acute gastroenteritis in Seoul, Korea...
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Molecular cloning and sequencing of rat Cdc42 GTPase cDNA

Han JS, Kim JH, Kim JG, Park JB, Noh DY, Lee KH

  • KMID: 755074
  • Exp Mol Med.
  • 2000 Sep;32(3):115-119.
Cdc42 is a member of the Rho family of small GTP-ase and plays an important role in intracellular signaling pathways regulating cell morphology, motility and stimulation of DNA...
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Overlapping Gene Mutations of Hepatitis B Virus in a Chronic Hepatitis B Patient with Hepatitis B Surface Antigen Loss during Lamivudine Therapy

Lee SY, Choi MS, Lee D, Lee JH, Koh KC, Paik SW, Yoo BC

Disappearance of hepatitis B surface antigens (HBsAg) in chronic hepatitis B usually indicates clearance of hepatitis B virus (HBV) infection. However, false HBsAg negativity with mutations in pre-S2 and 'a'...
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A case study of apple seed and grape allergy with sensitisation to nonspecific lipid transfer protein

Murad A, Katelaris , Baumgart K

Lipid transfer proteins can be an important cause of allergy given their stability and high degree of protein sequence homology. We describe the case of a child who developed two...
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Genomic organization and expression of parkin in Drosophila melanogaster

Bae YJ, Park KS, Kang SJ

  • KMID: 1093612
  • Exp Mol Med.
  • 2003 Oct;35(5):393-402.
We report here the isolation, characterization on genomic structure and expression of the D. melanogaster homolog of human parkin. The 2,122 bp parkin gene sequence contains six exons that form...
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Localization of Antigenic Sites at the Amino-terminus of Rinderpest Virus N Protein Using Deleted N Mutants and Monoclonal Antibody

Choi KS, Nah JJ, Ko YJ, Kang SY, Joo YS

  • KMID: 1089408
  • J Vet Sci.
  • 2003 Aug;4(2):167-173.
The nucleocapsid (N) protein of rinderpest virus (RPV) is highly conserved, immunogenic, and abundantly expressed during infection. Six antigenic sites (sites A, B, C, D, E and F), defined previously...
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Point mutation and polymorphism in Duchenne/Becker muscular dystrophy (D/BMD) patients

Chaturvedi LS, Mukherjee , Srivastava S, Mittal RD, Mittal B

  • KMID: 1457538
  • Exp Mol Med.
  • 2001 Dec;33(4):251-256.
Duchenne and Becker muscular dystrophies (D/BMD) are caused by mutations in the dystrophin gene. Two-thirds of patients have large intragenic deletions or duplications and the remaining one-third have point mutations,...
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