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Variation of nuclear and mitochondrial DNAs in Korean and Chinese isolates of Clonorchis sinensis

Lee SU, Huh S

We compared the DNA sequence difference of isolates of Clonorchis sinensis from one Korean (Kimhae) and two Chinese areas (Guangxi and Shenyang). The sequences of nuclear rDNA (18S, internal transcribed...
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Identification of parasite DNA in common bile duct stones by PCR and DNA sequencing

Jang JS, Kim KH, Yu JR, Lee SU

We attempted to identify parasite DNA in the biliary stones of humans via PCR and DNA sequencing. Genomic DNA was isolated from each of 15 common bile duct (CBD) stones...
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Ancient Mitochondrial DNA Analyses of Ascaris Eggs Discovered in Coprolites from Joseon Tomb

Oh CS, Seo M, Hong JH, Chai JY, Oh SW, Park JB, Shin DH

Analysis of ancient DNA (aDNA) extracted from Ascaris is very important for understanding the phylogenetic lineage of the parasite species. When aDNAs obtained from a Joseon tomb (SN2-19-1) coprolite in...
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Molecular Description of Macroorchis spinulosus (Digenea: Nanophyetidae) Based on ITS1 Sequences

Won EJ, Kim DG, Cho J, Jung BK, Kim MJ, Yun YW, Chai JY, Ryang DW

We performed a molecular genetic study on the sequences of 18S ribosomal RNA (ITS1 region) gene in 4-day-old adult worms of Macroorchis spinulosus recovered in mice experimentally infected with metacercariae...
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Subgenus classification of Acanthamoeba by riboprinting

Chung DI, Yu HS, Hwang MY, Kim TH, Kim TO, Yun HC, Kong HH

Subgenus classification of Acanthamoeba remains uncertain. Twenty-three reference strains of Acanthamoeba including 18 (neo)type-strains were subjected for classification at the subgenus level by riboprinting. PCR/RFLP analysis of 18S rRNA gene...
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Evaluation of taxonomic validity of four species of Acanthamoeba: A. divionensis, A. paradivionensis, A. mauritaniensis, and A. rhysodes, inferred from molecular analyses

Liu H, Moon EK, Yu HS, Jeong HJ, Hong YC, Kong HH, Chung DI

The taxonomy of Acanthamoeba spp., an amphizoic amoeba which causes granulomatous amoebic encephalitis and chronic amoebic keratitis, has been revised many times. The taxonomic validity of some species has yet...
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Occurrence and Molecular Identification of Giardia duodenalis from Stray Cats in Guangzhou, Southern China

Zheng G, Hu W, Liu Y, Luo Q, Tan L, Li G

The objective of this study was to genetically characterize isolates of Giardia duodenalis and to determine if zoonotic potential of G. duodenalis could be found in stray cats from urban...
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Keratitis by Acanthamoeba triangularis: Report of Cases and Characterization of Isolates

Xuan YH, Chung BS, Hong YC, Kong HH, Hahn TW, Chung DI

Three Acanthamoeba isolates (KA/E9, KA/E17, and KA/E23) from patients with keratitis were identified as Acanthamoeba triangularis by analysis of their molecular characteristics, a species not previously recognized to be a...
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Genotyping of Giardia duodenalis Isolates from Dogs in Guangdong, China Based on Multi-Locus Sequence

Zheng G, Alsarakibi M, Liu Y, Hu W, Luo Q, Tan L, Li G

  • KMID: 1792186
  • Korean J Parasitol.
  • 2014 Jun;52(3):299-304.
This study aimed to identify the assemblages (or subassemblages) of Giardia duodenalis by using normal or nested PCR based on 4 genetic loci: glutamate dehydrogenase (gdh), triose phosphate isomerase (tpi),...
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Molecular Characterization of Various Trichomonad Species Isolated from Humans and Related Mammals in Indonesia

Kamaruddin M, Tokoro M, Rahman MM, Arayama S, Hidayati A, Syafruddin D, Asih PB, Yoshikawa , Kawahara E

Trichomonad species inhabit a variety of vertebrate hosts; however, their potential zoonotic transmission has not been clearly addressed, especially with regard to human infection. Twenty-one strains of trichomonads isolated from...
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PCR Detection and Molecular Characterization of Pentatrichomonas hominis from Feces of Dogs with Diarrhea in the Republic of Korea

Kim YA, Kim HY, Cho SH, Cheun HI, Yu JR, Lee SE

Pentatrichomonas hominis is considered a commensal protozoan in the large intestine of a number of mammalian hosts, such as cats, dogs, and non-human primates. The resulting infections, which can induce...
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A Case of Plasmodium ovale Malaria Imported from West Africa

Kang Y, Yang J

Malaria is a parasitic infection caused by Plasmodium species. Most of the imported malaria in Korea are due to Plasmodium vivax and Plasmodium falciparum, and Plasmodium ovale infections are very...
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Molecular Differentiation of Schistosoma japonicum and Schistosoma mekongi by Real-Time PCR with High Resolution Melting Analysis

Kongklieng A, Kaewkong W, Intapan PM, Sanpool O, Janwan P, Thanchomnang T, Lulitanond V, Sri-Aroon , Limpanont , Maleewong

Human schistosomiasis caused by Schistosoma japonicum and Schistosoma mekongi is a chronic and debilitating helminthic disease still prevalent in several countries of Asia. Due to morphological similarities of cercariae and...
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Natural occurrence of Mycobacterium as an endosymbiont of Acanthamoeba isolated from a contact lens storage case

Yu HS, Jeong HJ, Hong YC, Seol SY, Chung DI, Kong HH

Recent in vitro studies have revealed that a certain Mycobacterium can survive and multiply within freeliving amoebae. It is believed that protozoans function as host cells for the intracellular replication...
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Genetic diversity of Acanthamoeba isolated from ocean sediments

Liu H, Ha YR, Lee ST, Hong YC, Kong HH, Chung DI

Genetic diversity of 18 Acanthamoeba isolates from ocean sediments was evaluated by comparing mitochondrial (mt) DNA RFLP, 18S rDNA sequences and by examining their cytopathic effects on human corneal epithelial...
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Comorbid Gastric Adenocarcinoma and Gastric and Duodenal Strongyloides stercoralis Infection: A Case Report

Seo AN, Goo YK, Chung DI, Hong Y, Kwon O, Bae HI

Strongyloides stercoralis can cause systemic infection, termed strongyloidiasis, and gastrointestinal ulcer disease in immunocompromised patients. However, to our knowledge, there are no reported cases of comorbid gastric adenocarcinoma and S....
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Morphologic and Genetic Evidence for Mixed Infection with Two Myxobolus Species (Myxozoa: Myxobolidae) in Gray Mullets, Mugil cephalus, from Korean Waters

Kim WS, Kim JH, Oh MJ

The present study was performed to trace the decisive evidence for mixed infection of 2 Myxobolus species, M. episquamalis and Myxobolus sp., in the gray mullet, Mugil cephalus, from Korean...
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Cryptosporidium suis Infection in Post-Weaned and Adult Pigs in Shaanxi Province, Northwestern China

Lin Q, Wang XY, Chen JW, Ding L, Zhao GH

Cryptosporidium spp., ubiquitous enteric parasitic protozoa of vertebrates, recently emerged as an important cause of economic loss and zoonosis. The present study aimed to determine the distribution and species of...
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Acanthamoeba sohi, n. sp., a pathogenic Korean isolate YM-4 from a freshwater fish

Im KI, Shin HJ

A new species of Acanthamoeba was isolated from a freshwater fish in Korea and tentatively named Acanthamoeba sp. YM-4 (Korean isolate YM-4). The trophozoites were 11.0-23.0 micrometer in length and...
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Molecular characterization of bacterial endosymbionts of Acanthamoeba isolates from infected corneas of Korean patients

Xuan YH, Yu HS, Jeong HJ, Seol SY, Chung DI, Kong HH

The endosymbionts of 4 strains of Acanthamoeba (KA/E9, KA/E21, KA/E22, and KA/E23) isolated from the infected corneas of Korean patients were characterized via orcein stain, transmission electron microscopic examination, and...
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