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Rapid Diagnosis of Duchenne Muscular Dystrophy DMD by Multiplex Polymerase Chain Reaction PCR using Uncultured Amniocytes

Lee KW, Lee SH

  • KMID: 1569613
  • Korean J Obstet Gynecol.
  • 2000 Oct;43(10):1713-1716.
No abstract available.
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Identification of Dermatophytes Using Multiplex Polymerase Chain Reaction

Kim JY, Choe YB, Ahn KJ, Lee YW

BACKGROUND: Multiplex polymerase chain reaction (PCR) allows more than two target DNA molecules to be amplified with more than two primers. This method is also useful for detecting various other...
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Prenatal Diagnosis of Duchenne Muscular Dystrophy (DMD) by Multiplex Polymerase Chain Reaction (PCR) with Chorionic Villi

Lee KW, Yoon SW, Chang WS, Kim JY, Lee SH, Lee SJ, Kim SD, Han JS

  • KMID: 2269871
  • Korean J Obstet Gynecol.
  • 1999 Sep;42(9):2028-2032.
OBJECTIVE: To evaluate the efficacy of prenatal multiplex PCR with chorionic villi, in the case of family history of DMD due to exon deletion. METHODS: DNA was extracted when cells'...
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Enhanced detection and serotyping of Streptococcus pneumoniae using multiplex polymerase chain reaction

Ahn JG, Choi SY, Kim DS, Kim KH

PURPOSE: Methods for quick and reliable detection of Streptococcus pneumoniae are needed for the diagnosis of pneumococcal disease and vaccine studies. This study aimed to show that sequential multiplex polymerase...
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Comparison of the Ability of Multiplex and Singleplex PCR to Detect Human Respiratory Viruses

Kim S, Eom KW, Cho CR, Um TH

BACKGROUND: The use of the multiplex polymerase chain reaction (PCR) technique for respiratory viruses has become popular in Korea owing to its convenience and sensitivity. However, concerns remain with regard...
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Results of Microorganism Detection by Multiplex Polymerase Chain Reaction in Patients with Chronic Pelvic Pain Syndrome

Seo KI, Hwang JC, Kim TW, Lee SH, Park SY, Roh SH

  • KMID: 2314746
  • Korean J Urol.
  • 2009 Nov;50(11):1120-1124.
PURPOSE: The aim of this study was to evaluate the results of multiplex polymerase chain reaction (PCR) in patients with chronic pelvic pain syndrome (CPPS) and the significance of microorganisms...
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Identification of Candida Species by Multiplex Polymerase Chain Reaction

Lee MK, Kim HR, Lee YJ

  • KMID: 2224724
  • Korean J Clin Microbiol.
  • 2006 Oct;9(2):119-124.
BACKGROUND: Polymerase chain reacation (PCR)-based methods have been described for rapid detection and identification of Candida spp. Multiplex PCR assay was developed using internal transcribed spacers and topoisomerase II gene...
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Detection and identification of Mycoplasma species by multiplex PCR in cats

Lee HA, Moon MN, Won JS, Im EK, Yoo JH, Kim O

Mycoplasma (M.) felis and M. canis is related with pneumonia or conjunctivitis in domestic cats and several diseases in a variety of other animals, including lower respiratory tract disease or...
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Multiplex PCR Assay for Identification of Mycobacterial Species Isolated from Liquid Cultures

Kee SJ, Kim SM, Kim SH, Shin MG, Shin JH, Suh SP, Ryang DW

We developed a 2-step multiplex polymerase chain reaction (PCR) assay to rapidly identify the most common mycobacterial species isolated from positive liquid cultures as follows. In the first step, a...
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Direct detection of hemophilia B F9 gene mutation using multiplex PCR and conformation sensitive gel electrophoresis

Yoo KY, Kim HJ, Lee KC

PURPOSE: The F9 gene is known to be the causative gene for hemophilia B, but unfortunately the detection rate for restriction fragment length polymorphism-based linkage analysis is only 55.6%. Direct...
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Detection of Cryptic Microorganisms in Patients with Chronic Prostatitis by Multiplex Polymerase Chain Reaction

Kim TH, Kim TH, Kim HR, Lee MK, Myung SC, Kim YS

PURPOSE: Chronic prostatitis frequently occurs in men of all ages. Recent studies suggest that fastidious microorganisms may play a role in chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS). The aim of...
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Development of a multiplex PCR to identify Salmonella, Leptospira and Brucella species in tissue samples

Lam TQ, Yoon BI, Hahn TW

  • KMID: 1435817
  • Korean J Vet Res.
  • 2012 Jun;52(2):75-82.
We have developed and optimized a multiplex polymerase chain reaction (mPCR) for simultaneous detection of Brucella, Salmonella and Leptospira with high sensitivity and specificity. Three pairs of oligonucleotide primers were...
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Supraclavicular BCG Lymphadenitis Noted at 21 Months after BCG Vaccination Confirmed by a Molecular Method

Lee MH, Chae MH, Park KU, Cho HK

  • KMID: 1720817
  • Korean J Pediatr Infect Dis.
  • 2014 Aug;21(2):139-143.
Bacille Calmette-Guerin (BCG) lymphadenitis is the most common complication of BCG vaccination. It commonly occurs in infants aged
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Utilization of the respiratory virus multiplex reverse transcription-polymerase chain reaction test for adult patients at a Korean tertiary care center

Ahn MY, Choi SH, Chung JW, Kim HR

BACKGROUND/AIMS: Respiratory viruses (RVs) are considered to be important respiratory pathogens in adult patients, and the multiplex reverse transcription-polymerase chain reaction (RT-PCR) test is used frequently in adult patients with...
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A multiplex quantitative real-time polymerase chain reaction panel for detecting neurologic pathogens in dogs with meningoencephalitis

Han JI, Chang DW, Na KJ

Meningoencephalitis (ME) is a common inflammatory disorder of the central nervous system in dogs. Clinically, ME has both infectious and non-infectious causes. In the present study, a multiplex quantitative real-time...
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Study of M.Tuberculosis and Non-Tuberculous Mycobacterium in Subacute Necrotizing Lymphadenitis Using Multiplex PCR

Cho MS, Han WS

OBJECTIVES: Subacute necrotizing lymphadenitis is a self-limiting disorder that typically affects cervical lymph nodes of young women. Although autoimmune reaction and a viral etiology have been suggested, the definite cause...
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Identification of Mycobacterium Species by Multiplex PCR-Restriction Fragment Length Polymorphism Assay

Seo YH, Kim W, Ann JY, Keum DG

  • KMID: 2248727
  • Korean J Infect Dis.
  • 1999 Apr;31(2):148-152.
BACKGROUND: Recently the clinical significance of several mycobacterial species has been increased and there is a growing need to identify mycobacteria to the species level. We evaluated multiplex polymerase chain...
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The Utility of the Multiplex Reverse Transcriptase-Polymerase Chain Reaction Assay in the Detection of Hematologic Malignancies

Kim MJ, Cho SY, Lee WI, Park TS, Lee HJ

No abstract available.
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Streptococcus pneumoniae Type Determination by Multiplex Polymerase Chain Reaction

Yun KW, Cho EY, Hong KB, Choi EH, Lee HJ

The purpose of this study was to develop pneumococcal typing by multiplex PCR and compare it with conventional serotyping by quellung reaction. Pneumococcal strains used in this study included 77...
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A Case of Vibrio cholerae non-O1/non-O139 Gastroenteritis

Lee KE, Cho JH

  • KMID: 1559955
  • Korean J Clin Microbiol.
  • 2002 Sep;5(2):139-142.
Vibrio cholerae is an autochthonous inhabitant of estuarine and seawater environment and is a facultative pathogen for humans. V. cholerae non-O1/non-O139 strains are associated with gastroenteritis, septicemia and/or extraintestinal infections....
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