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Isolation and identification of Escherichia coli O157:H7 using different detection methods and molecular determination by multiplex PCR and RAPD

Kim JY, Kim SH, Kwon NH, Bae WK, Lim JY, Koo HC, Kim JM, Noh KM, Jung WK, Park KT, Park YH

  • KMID: 1106069
  • J Vet Sci.
  • 2005 Mar;6(1):7-19.
Escherichia coli O157:H7 is recognized as a significant food-borne pathogen, so rapid identification is important for food hygiene management and prompt epidemiological investigations. The limited prevalence data on Shiga toxin-producing...
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Evaluation of PCR inhibitory effect of enrichment broths and comparison of DNA extraction methods for detection of Salmonella Enteritidis using real-time PCR assay

Hyeon JY, Hwang IG, Kwak HS, Park C, Choi IS, Seo KH

The best enrichment broth and DNA extraction scheme was determined for rapid and sensitive detection of Salmonella Enteritidis in steamed pork using real-time PCR. The inhibitory effect of commonly used...
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A multiplex real-time PCR for differential detection and quantification of Salmonella spp., Salmonella enterica serovar Typhimurium and Enteritidis in meats

Lee SH, Jung BY, Rayamahji , Lee HS, Jeon WJ, Choi KS, Kweon CH, Yoo HS

Salmonella (S.) Typhimurium and S. Enteritidis are the major causative agents of food-borne illnesses worldwide. Currently, a rapid detection system using multiplex real-time polymerase chain reaction (PCR) has been applied...
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