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Antigen analysis of Toxoplasma gondii lysate and excretory-secretory materials by enzyme-linked immunoelectrotransfer blot(EITB)

Ahn MH, Son HJ, Leem MH, Min DY

Recently, the importance of toxoplasmosis is raised as a complication in immunosuppressed or AIDS patients. Our study focused on the identification of a variety of Toxoplasma antigens by immunoblotting. Rabbits...
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Protein composition and antigenicity of the tegument from Paragonimus westermani

Kim SI, Cho SY

To ascertain that tegument of Paragonimus westermani has specific antigenic proteins, the tegumental fraction was isolated from 10-month-old worms by 0.1% digitonin solution, and subjected to SDS-PAGE and immunoblot. Component...
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Isolation and partial characterization of cysteine proteinase from sparganum

Song CY, Choi DH, Kim TS, Lee SH

A proteolytic enzyme was purified from the tissue extract of spargana (plerocercoids of Spirometra erinacei) by DEAE-Trisacryl M ion exchange chromatography and thiopropyl-sepharose affinity chromatography resulted in a 21-fold purification....
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Antigenic protein fractions of Metagonimus yokogawai reacting with patient sera

Lee SC, Chung YB, Kong Y, Kang SY, Cho SY

Antibody test is sometimes necessary for the diagnosis of acute human metagonimiasis because eggs may not be detected in stool. The antibody test (ELISA) was evaluated for its significance by...
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Comparative antigen analysis of Trichomonas vaginalis by enzyme-linked immunoelectrotransfer blot technique

Min DY, Leem MH, Kim JM, Choi YK

Analysis of six isolates of Trichomonas vaginalis was carried out with sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and enzyme-linked immunoelectrotransfer blot (EITB). Trichloroacetic acid-treated antigens of the 6 isolates revealed...
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Identification of surface antigen of Trichomonas vaginalis

Min DY, Leem MH, Ahn MH, Ryu JS

Plasma membrane proteins of a Korean isolate of Trichomonas vaginalis HY-1 were fractionated for antigen analysis. Homogenates of T. vaginalis were fractionated by the differential centrifugation using sucrose step-gradient method....
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Changes of IgM and IgG antibody levels in experimental rabbit anisakiasis as observed by ELISA and SDS-PAGE/immunoblot

Yang HJ, Cho YJ, Paik YH

Antibody changes in experimental anisakiasis were observed in 10 rabbits which were infected each with 10 Anisakis simplex larvae. The sera were collected before and on the 6th to the...
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Antibody changes in paragonimiasis patients after praziquantel treatment as observed by ELISA and immunoblot

Cho SY, Kim SI, Kang SY, Kong Y, Han SK, Shim YS, Han YC

To observe antibody changes after praziquantel treatment in paragonimiasis, a total of 46 serum samples from 13 serologically diagnosed patients was collected for 4-28 months. The specific antibody (IgG) levels...
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Analysis of antigenic specificities of Paragonimus westermani developmental stages using immunoblot technique

Joo KH, Hong SC, Chung MS, Rim HJ

Serodiagnosis of parasitic infections is widely used, since parasites or their eggs are not always detected by ordinary methods. The sensitive tests such as ELISA are highly dependent on the...
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