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Cloning and sequencing of p33 in a Korean isolate of Theileria sergenti

Kang SW, Choi EJ, Kweon CH

The gene encoding the 33 kDa piroplasm surface protein of Theileria sergenti isolated in Korea was cloned and the nucleotide sequence was determined by dideoxy chain termination method. The cloned...
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Genetic characteristics of the Korean isolate KI-1 of Toxoplasma gondii

Lin A, Shin EH, Kim TY, Park JH, Guk SM, Chai JY

Toxoplasma gondii tachyzoites were isolated from an ocular patient in the Republic of Korea and maintained in the laboratory (designated KI-1). In the present study, its genotype was determined by...
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Identical small subunit ribosomal RNA gene nucleotide sequence of bovine Theileria isolates (Korea and Japan) and Theileria buffeli (Marula, Kenya)

Chae JS, Kwon OD, Holman PJ, Waghela SD, Wagner GG, Lee JM

Small subunit ribosomal RNA (SSU rRNA) gene nucleotide sequences of bovine Theileria isolates from Korea (KLS and KCB) and Japan (JHS) were determined. The genes from each isolate were amplified...
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Identification of differentially expressed cDNAs in Acanthamoeba culbertsoni after mouse brain passage

Han KL, Lee J, Kim DS, Park SJ, Im KI, Yong TS

Free-living amoebae of the genus Acanthamoeba are causative agents of granulomatous amebic encephalitis and amebic keratitis. Because the virulence of Acanthamoeba culbertsoni cultured in the laboratory is restored by consecutive...
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In vivo determination of the gap2 gene promoter activity in Giardia lamblia

Yang HW, Kim J, Yong TS, Park SJ

A shuttle vector for Escherichia coli and Giardia lamblia was modified to produce a reporter plasmid, which monitors the expression of prescribed gene in G. lamblia by measuring its luciferase...
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Isolation and characterization of a cDNA encoding a mammalian cathepsin L-like cysteine proteinase from Acanthamoeba healyi

Hong YC, Hwang MY, Yun HC, Yu HS, Kong HH, Yong TS, Chung DI

We have cloned a cDNA encoding a cysteine proteinase of the Acanthamoeba healyi OC-3A strain isolated from the brain of a granulomatous amoebic encephalitis patient. A DNA probe for an...
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Usefulness of the recombinant liver stage antigen-3 for an early serodiagnosis of Plasmodium falciparum infection

Lee HW, Moon SU, Ryu HS, Kim YJ, Cho SH, Chung GT, Lin K, Na BK, Kong Y, Chung KS, Kim TS

In order to develop tools for an early serodiagnosis of Plasmodium falciparum infection, we evaluated the usefulness of P. falciparum liver stage antigen-3 (LSA-3) as a serodiagnostic antigen. A portion...
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Analysis of polymorphic region of GAM-1 gene in Plasmodium vivax Korean isolates

Kho WG, Chung JY, Hwang UW, Chun JH, Park YH, Chung WC

The identification, characterization and quantification of Plasmodium sp. genetic polymorphism are becoming increasingly important in the vaccine development. We investigated polymorphism of Plasmodium vivax GAM-1 (PvGAM-1) gene in 30 Korean...
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The MAK16 Gene of Entamoeba histolytica and Its Identification in Isolates from Patients

Crisostomo-Vazquez MdP, Marevelez-Acosta VA, Flores-Luna A, Jimenez-Cardoso E

To identify sequences of Entamoeba histolytica associated with the development of amebic liver abscess (ALA) in hamsters, subtractive hybridization of cDNA from E. histolytica HM-1:IMSS under 2 growth conditions was...
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