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A Novel Nicotinamide Adenine Dinucleotide Correction Method for Mitochondrial Ca2+ Measurement with FURA-2-FF in Single Permeabilized Ventricular Myocytes of Rat

Lee JH, Ha JM, Leem CH

Fura-2 analogs are ratiometric fluoroprobes that are widely used for the quantitative measurement of [Ca2+]. However, the dye usage is intrinsically limited, as the dyes require ultraviolet (UV) excitation, which...
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PKC-Independent Stimulation of Cardiac Na+/Ca2+ Exchanger by Staurosporine

Kang TM

[Ca2+]i transients by reverse mode of cardiac Na+/Ca2+ exchanger (NCX1) were recorded in fura-2 loaded BHK cells with stable expression of NCX1. Repeated stimulation of reverse NCX1 produced a long-lasting...
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Mepivacaine-induced intracellular calcium increase appears to be mediated primarily by calcium influx in rat aorta without endothelium

Ok SH, Kwon SC, Kang S, Choi MJ, Sohn JT

BACKGROUND: Mepivacaine induces contraction or decreased blood flow both in vivo and in vitro. Vasoconstriction is associated with an increase in the intracellular calcium concentration ([Ca2+]i). However, the mechanism responsible...
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Role of Cytosolic Ca2+ in the Enhancement and Inhibition of the Proliferation of Vascular Smooth Muscle Cells

Yoo HJ, Ihm SH

  • KMID: 2338172
  • J Korean Geriatr Soc.
  • 2000 May;4(1):28-34.
Background : Vascular smooth muscle cells(VSMCs) proliferation is an important process in the pathogenesis and progression of atherosclerosis, Calcium is considered an important regulator of cellular proliferation. The major mechanism...
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Changes in Cytosolic Ca2+ Concentration of Single Rabbit Coronary Artery Smooth Muscle Cell during Ischemic Cardioplegic Period

Lee YH, Choi GB, Kim ST, Kang BS

BACKGROUND: No-reflow is a specific type of vascular damage occuring when removal of coronary occlusion dose not lead to restoration of coronary flow. There are three major explanations for the...
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Effects of Ca2+ Channel Blockers on Expression of L-type Voltage Dependent Ca2+ Channels in Vascular Smooth Muscle Cells Obtained from the Aorta of Diabetic Rats

Yoo HJ

  • KMID: 2338249
  • J Korean Geriatr Soc.
  • 2003 Jun;7(2):95-100.
BACKGROUND: The proliferation of vascular smooth muscle cells(VSMCs) is an important process in the pathogenesis of atherosclerosis. VSMCs proliferation is closedly related to the cytosolic calcium flux via L-type voltage...
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Reperfusion Injury in Glial Cells: The Phenomenon and Mechanism of "Calcium Paradox"

Kim MH, Lee BD, Kim YH, Shin BH

BACKGROUND: Mechanisms of secondary injury(post-ischemic injury) in the central nervous system have recently reported in a vast of amount of experiments. Among many factors which give rise to post-ischemic neuronal...
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Effects of glucosamine on Ca2+ signaling and K+ channel currents in T lymphocytes

Suh EY, Pang B, Kim SJ, Kim WK

  • KMID: 1458431
  • Korean J Med.
  • 2010 Nov;79(5):536-542.
BACKGROUND/AIMS: Glucosamine is widely taken as a functional food, and some studies reported its anti-inflammatory effects. K+ channels and intracellular signal play important roles in the activation of immune cells...
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Early Detection of Anesthesia Machine Malfunction with Capnography during General Endotrachial Anesthesia

Lee SK, Kim JS, Kang SK, Lee JL

BACKGROUND: Mechanisms of secondary injury (post-ischemic injury) in the central nervous system have cently reported in a vast of amount of experiments. Among many factors which give rise to post-ischemic...
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Effects of Estrogen on Intracellular Calcium-Related T-Lymphocyte Function

Kim YY, Kim H, Ku SY, Suh CS, Kim SH, Choi YM, Kim SJ

Regulation of immune cell function is an important in the field of hormone-related tissue engineering and regenerative medicine. In this sense, hormonal regulation of immune cell function is a critical...
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ATP-induced Calcium Mobilization and Proliferation in Cervical Cancer Cells

An WS, Le GH

  • KMID: 2261415
  • Korean J Obstet Gynecol.
  • 1999 Jun;42(6):1332-1338.
PURPOSE: Nucleotides such as ATP can act as extracellular effector molecules by interaction with specific cellular receptors. To know the role of nucleotides in cervical cancer cells, we observed the...
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Effect of Sphingosine-1-Phosphate on Intracellular Free Ca2+ in Cat Esophageal Smooth Muscle Cells

Lee DK, Min YS, Yoo SS, Shim HS, Park SY, Sohn UD

A comprehensive collection of proteins senses local changes in intracellular Ca²⁺ concentrations ([Ca²⁺](i) and transduces these signals into responses to agonists. In the present study, we examined the effect of...
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Mechanism of Acetylcholine-induced Endothelium-dependent Relaxation in the Rabbit Carotid Artery by M3-receptor Activation

Song YJ, Kwon SC

  • KMID: 1611489
  • Korean J Physiol Pharmacol.
  • 2004 Dec;8(6):313-317.
The present study were designed to characterize the action mechanisms of acetylcholine (ACh) -induced endothelium-dependent relaxation in arteries precontracted with high K (70 mM). For this, we simultaneously measured both...
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Role of Cytosolic Calcium Ion and Expression of Proliferation Responsive Gene of Cultured Medial Smooth Muscle Cells Obtained from rat Aortas with Atherosclerotic Changes Induced by High Cholesterol Feeding

Yoo HJ

  • KMID: 2188724
  • J Korean Geriatr Soc.
  • 2000 Dec;4(4):251-256.
BACKGROUND: Vascular smooth muscle cells (VSMCs) proliferation is an important process in the pathogenesis of atherosclerosis. VSMCs proliferation is closedly related to the cytosolic calcium flux via L-type voltage dependent...
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The Effects of DTBNP on Intracellular Ca2+ Signaling in Cultured Bovine Aortic Endothelial Cells

Park SJ, Kim BJ, Zhu MH, So I, Kim KW

  • KMID: 2071617
  • Korean J Physiol Pharmacol.
  • 2005 Dec;9(6):341-346.
The mechanism underlying oxidant-induced intracellular Ca2+ ([Ca2+]i) increase was studied in cultured bovine aortic endothelial cells (BAECs) using fura-2 AM. In the presence of 2 mM extracellular Ca2+, the application...
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Characteristics of Ca2+ stores in rabbit cerebral artery myocytes

Kim SJ, Kim JK, So I, Suh SH, Lee SJ, Kim KW

  • KMID: 1849839
  • Korean J Physiol Pharmacol.
  • 1998 Jun;2(3):313-322.
In a myocyte freshly isolated from rabbit cerebral artery, the characteristics of Ca2+ release by histamine or caffeine were studied by microspectrofluorimetry using a Ca2+-binding fluorescent dye, fura-2. Histamine (5 micrometer) or caffeine...
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Effects of Noradrenaline on the Membrane Potential of Prostatic Neuroendocrine Cells of Rat

Kim JH, Shin SY, Uhm DY, Kim SJ

  • KMID: 1607354
  • Korean J Physiol Pharmacol.
  • 2003 Feb;7(1):47-52.
The prostate gland contains numerous neuroendocrine cells that are believed to influence the function of the prostate gland. Our recent study demonstrated the expression of both alpha 1- and alpha...
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Buffering Contribution of Mitochondria to the [Ca2+]i Increase by Ca2+ Influx through Background Nonselective Cation Channels in Rabbit Aortic Endothelial Cells

Kim YC, Lee SJ, Kim KW

  • KMID: 2285307
  • Korean J Physiol Pharmacol.
  • 2005 Feb;9(1):29-35.
To prove the buffering contribution of mitochondria to the increase of intracellular Ca2+ level ([Ca2+]i) via background nonselective cation channel (background NSCC), we examined whether inhibition of mitochondria by protonophore...
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Effects of NaOCl on Neuronal Excitability and Intracellular Calcium Concentration in Rat Spinal Substantia Gelatinosa Neurons

Lee HI, Park AR, Chun SW

  • KMID: 1431123
  • Int J Oral Biol.
  • 2013 Mar;38(1):5-12.
Recent studies indicate that reactive oxygen species (ROS) can act as modulators of neuronal activity, and are critically involved in persistent pain primarily through spinal mechanisms. In this study, we...
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Modulation of Pacemaker Ca2+ Activity by Serotonin in the Cultured Interstitial Cells of the Cajal Clusters Isolated from Mouse Small Intestine

Sinn DH, Rhee PL, Son HJ, Kim JJ, Lee JY, So IS

  • KMID: 2307492
  • Korean J Neurogastroenterol Motil.
  • 2006 Dec;12(2):151-156.
BACKGROUND/AIMS: Interstitial cells of Cajal (ICCs) are considered to be pacemaker cells in the gastrointestinal tracts and Ca2+ oscillation in ICCs is intimately linked to the pacemaker function. Although serotonin...
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