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Study of ethidium bromide interaction peculiarities with DNA

Vardevanyan PO, Antonyan A, Manukyan G, Karapetyan AT

  • KMID: 1457531
  • Exp Mol Med.
  • 2001 Dec;33(4):205-208.
The helix-coil transition of DNA-ethidium bromide complexes in an interval of ionic strength of 2.0 x 10(-3) M < or = muNa+ < or = 2.0 x 10(-2) M has...
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Cooperative binding interaction of ethidium with allosteric DNA

Suh D

  • KMID: 755044
  • Exp Mol Med.
  • 1999 Sep;31(3):151-158.
The specific association of drugs with deoxyoligonucleotides, containing a B-Z junction between left-handed Z-DNA and right-handed B-DNA, was examined by fluorescence and circular dichroism (CD) technique. Ethidium was chosen for a simple DNA...
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The activity of antimelanocyte autoantibodies in vitiligo patients

Park YK, Hann SK, Song MS, Yoon JK, Kim HI

  • KMID: 2303626
  • Korean J Dermatol.
  • 1991 Jun;29(3):391-398.
Several observation suggest that the antimelanocyte autoantibodies could play a role in melanocyte destruction. Some experiments indicate that melanocyte antibodies from patients with vitiligo can kill melanocyte in vitro. In...
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The Optimal Conditions of Chromosomal Analysis in Peripheral Blood

Son HG, Lee YI, Jung BJ, Lee SH, Kang SJ

  • KMID: 2262162
  • Korean J Obstet Gynecol.
  • 2001 Jan;44(1):5-16.
OBJECTIVE: To find out the optimal conditions of human chromosomal analysis protocol in peripheral blood sample. METHODS: The experiments were made with the variations of phytohaemagglutinin, colcemid, ethidium bromide concentration and...
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Effects of Antioxidants on Ethidium Bromide-induced Inhibition of Insulin Secretion in Rat Pancreatic Islets

Kim CH, Kim CH, Park HK, Suh KI, Lee KU

  • KMID: 2177840
  • J Korean Diabetes Assoc.
  • 2002 Jun;26(3):179-187.
BACKGROUND: It was recently shown that mitochondrial function in pancreatic beta-cells is essential in nutrient-stimulated insulin secretion. The inhibition of mitochondrial DNA (mtDNA) transcription by ethidium bromide (EtBr) has been...
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Comparison between Real-Time PCR and Agarose Gel Electrophoresis for DNA Quantification

Lee MK, Kim HR

BACKGROUND: Real-time polymerase chain reaction (PCR) is generally regarded as a very accurate and time-saving method, but it is expensive to run. We evaluated the reliability of an inexpensive and...
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Mutation in p53 Tumor Suppressor Gene in Gestational Trophoblastic Neoplasia

Kim SC, Moon HS, Kim HR

  • KMID: 2083712
  • Korean J Gynecol Oncol Colposc.
  • 1997 Dec;8(4):339-349.
This study attempted to determine the status of tumor suppressor gene p53 in gestational trophoblstic neoplasia. In this study, 19 cases of hydatidiform moles and two choriocarcinoma cell lines(JAR, JEG-3)...
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Genotyping of Diego Blood Group System by Use of Polymerase Chain Reaction and Nae I Restriction Enzyme

Kim DA, Kim TY, Choi TY

  • KMID: 2225861
  • Korean J Clin Pathol.
  • 1999 Apr;19(2):246-251.
BACKGROUND: The Diego blood group system consists of two independent pairs of antigens, Dia and Dib. Immunization to Dia or Dib is clinically significant, because anti-Dia and anti-Dib may cause...
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The Duffy Blood Group Genotypes in Asian Populations

Lim CS, Kim YK, Lee KN

  • KMID: 1909649
  • Korean J Blood Transfus.
  • 2007 Dec;18(3):145-151.
BACKGROUND: Duffy phenotypes vary widely among different ethnic groups and populations from distinct locations. Several assays for genotyping Duffy blood groups have recently been developed, and PCR-restriction fragment length polymorphism...
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Apoptotic process is involved in the L-glutamate-induced PC12 cell death

Sung KW, Jung KH, Kim SY, Kang JH, Lee SB

  • KMID: 2285188
  • Korean J Physiol Pharmacol.
  • 1997 Dec;1(6):699-705.
Although it is known that neuronal cell death during development occurs by apoptosis, the mechanisms underlying excitatory amino acid-induced neuronal cell death remain poorly understood. In this study we have examined the mechanism...
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Roles of Dopamine in Proliferation of Gastric-Cancer Cells

Jeong HJ, Chae HD

  • KMID: 2372824
  • J Korean Gastric Cancer Assoc.
  • 2006 Sep;6(3):132-138.
PURPOSE: Dopamine is a neurotransmitter, but in the GIT, the roles of dopamine are a regulator of epithelial cell proliferation, an endogenous protective factor, and a regulator of stomach cancer...
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Analysis of the alleles and the Genotypes at the VNTR D1S80 Locus in Koreans

Park JT, Juhng SW

  • KMID: 2082608
  • Korean J Leg Med.
  • 1997 May;21(1):75-86.
Alleles and genotype frequencies and its distribution pattern for the highly polymorphic D1S80 locus were determined in a Korean population sample, especially in Kwangju and Chonnam, by using PCR followed...
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One-Stage Polymerase Chain Reaction for the Comprehensive Detection of Type D Retrovirus Provial DNA

Jeong YS

  • KMID: 2204562
  • J Korean Soc Virol.
  • 1997 Mar;27(1):19-27.
To develop the polymerase chain reaction (PCR) for the detection of type D simian retrovirus (SRV) infection, an oligonucleotide primer pair was designed to hybridize to the sequences within euv...
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Environments of ethidium binding to allosteric Dna: II. Accessibility, mobility and mode of binding

Suh DC

  • KMID: 755100
  • Exp Mol Med.
  • 2000 Dec;32(4):204-209.
DNA binding compounds were previously shown to bind to the right-handed DNA forms and hybrid B-Z forms in a highly cooperative manner and indicate that structural specificity plays...
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Frepuency of the Angiotensin - Converting Enzyme (ACE) Gene Polymorphism in the General Population and the Elite Endurance Students in Korea

Choung HJ, Yoon SR, Choi SK

  • KMID: 1536937
  • J Genet Med.
  • 1999 Dec;3(1):11-14.
Recintly it was reported that Insertion / Deletion polymorphism in the gene coding for Angiotensin Converting Enzyme (ACE) is asscoiated with human capacity for physical performance. This study was performed...
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Apolipoprotein E polymorphisms in the development of hypertensive diseases of pregnancy in Korean women

Nam H, Kang JH, Jun JK

  • KMID: 2077888
  • Korean J Obstet Gynecol.
  • 2008 Jul;51(7):716-724.
OBJECTIVE: Hypertensive disorder in pregnancy is one of the most serious obstetrical complications. Apolipoprotein E (Apo E) genotype is polymorphic and recently it was reported to be associated with preeclampsia....
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Detection of nasopharyngeal carriages in children by multiplex reverse transcriptase-polymerase chain reaction

Shin JH, Han HY, Kim SY

PURPOSE: The aim of this study was to determine the prevalence of asymptomatic nasopharyngeal carriages in children using a multiplex reverse transcriptase-polymerase chain reaction (mRT-PCR) assay kit. METHODS: We obtained...
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Expression of Dermal Keratinocyte Growth Factor mRNA in Cutaneous Squamous Cell Carcinoma and Basal Cell Carcinoma

Cun TJ, Whang KK, Hong CK, Ro BI, Seo SJ

  • KMID: 1863721
  • Korean J Dermatol.
  • 2000 Mar;38(3):352-357.
BACKGROUND: Human keratinocyte growth factor(KGF) is an epithelial cell specific mitogen which is secreted by normal stromal fibroblast. In skin, KGF has been shown to stimulate keratinocyte proliferation. OBJECTIVE: In the present...
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Diagnosis of Tuberculous Lymphadenitis by Polymerase Chain Reaction

Seo YH, Kim W, Choi TY

  • KMID: 2248638
  • Korean J Infect Dis.
  • 1997 May;29(3):219-224.
BACKGROUND: Tuberculous lymphadenitis is the commonest form of extrapulmonary tuberculosis. The laboratory diagnosis of tuberculous lymphadenitis is based on the traditional method of the acid-fast stain and culture of discharge...
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Expression of the FHIT gene Located in Chromosome 3p14.2 in Human Lung Cancer Cell Lines

Kim CH, Yoo CG, Lee CT, Han SK, Shim YS, Kim YW

  • KMID: 2318161
  • Tuberc Respir Dis.
  • 1998 Oct;45(5):984-991.
BACKGROUND: The 3p deletions has been shown to be the most frequent alteration in lung cancers, strongly suggesting the presence of at least one tumor suppressor gene in this chromosomal...
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