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SIRT1 Inhibits p53 but not NF-kappaB Transcriptional Activity during Differentiation of Mouse Embryonic Stem Cells into Embryoid Bodies

Lhee SJ, Song EK, Kim YR, Han MK

  • KMID: 1735406
  • Int J Stem Cells.
  • 2012 Nov;5(2):125-129.
BACKGROUND AND OBJECTIVES: SIRT1, a histone diacetylase, modify transactivation function of various transcription factor including p53 and NF-kappaB. p53 and NF-kappaB is involved in in vitro differentiation of mouse embryonic...
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Assessment of Developmental Toxicants using Human Embryonic Stem Cells

Hong EJ, Jeung EB

  • KMID: 2317839
  • Toxicol Res.
  • 2013 Dec;29(4):221-227.
Embryonic stem (ES) cells have potential for use in evaluation of developmental toxicity because they are generated in large numbers and differentiate into three germ layers following formation of embryoid...
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Establishment of Embryonic Stem Cell Line from Isolated Blastomeres from Mouse Preimplantation Embryos

Lim CK, Sung JH, Choi HW, Cho JW, Shin MR, Jun JH

  • KMID: 1511387
  • Korean J Fertil Steril.
  • 2006 Mar;33(1):25-34.
OBJECTIVE: The aim of this study was to investigate whether embryonic stem (ES) cells can be established from isolated blastomeres of mouse embryos. METHODS: Blastomeres were separated from mouse (C57Bl/6J) 2-...
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Enhanced Cardiomyogenic Differentiation of P19 Embryonal Carcinoma Stem Cells

Yang J, Ko SJ, Kim BS, Kim HS, Park S, Hong D, Hong SW, Choi JH, Park CY, Choi SC, Hong SJ, Lim DS

BACKGROUND AND OBJECTIVES: We investigated the effects of different concentrations of serum, 5-azacytidine, and culture time on the cardiomyogenic differentiation of P19 embryonal carcinoma stem cells in the course of...
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Functional Comparison of Human Embryonic Stem Cells and Induced Pluripotent Stem Cells as Sources of Hepatocyte-Like Cells

Jeong J, Kim KN, Chung MS, Kim HJ

Pluripotent stem cells can differentiate into many cell types including mature hepatocytes, and can be used in the development of new drugs, treatment of diseases, and in basic research. In...
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MHC Expression in Human Embryonic Stem Cells and Embryoid Bodies

Kim D, Kim JY, Lee EM, Song M, Yang J, Lee JW, Lee JP, Oh SK, Kim KS, Moon SY, Lee JS, Ahn C

  • KMID: 2003630
  • J Korean Soc Transplant.
  • 2003 Jun;17(1):26-33.
PURPOSE: Human embryonic stem (ES) cell is pluripotent cell derived from a group of cells called the inner cell mass and has the ability to reproduce itself for long periods...
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Transduction of eGFP Gene to Human Embryonic Stem Cells and Their Characterization

Kim YY, Ku SY, Park YB, Oh SK, Moon SY, Choi YM

  • KMID: 2288259
  • Korean J Reprod Med.
  • 2009 Dec;36(4):283-292.
OBJECTIVE: Human embryonic stem cells (hESCs) can proliferate indefinitely and differentiate into all kinds of cell types in vitro. Therefore, hESCs can be used as a cell source for cell-based...
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Isoflurane decreases apoptosis of human embryonic stem cell-derived cardiac progenitor cell under oxidative stress

Koo BW, Kim JH, Han SH, Kim MH, Baik JS

  • KMID: 1795955
  • Anesth Pain Med.
  • 2013 Jul;8(3):175-180.
BACKGROUND: Despite the great potential of human embryonic stem cell (hESC)-derived cardiac progenitor cells (CPCs) in the cardiac cell transplantation, the low graft survival still remains as one of the...
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Establishment and Characterization of Multipotent Germ Line Stem Cells (MGSCs) from Neonatal Mouse Testis

Han SC, Song H, Jun JH

  • KMID: 1483347
  • Korean J Reprod Med.
  • 2008 Mar;35(1):39-48.
OBJECTIVE: The aim of this study was to investigate whether multipotent germline stem cells (MGSCs) can be established from neonatal mouse testis. METHODS: Various cells containing MGSCs were collected from neonatal...
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Human Amniotic Fluid Cells Support Expansion Culture of Human Embryonic Stem Cells

Kim HS, Seol HW, Ahn HJ, Oh SK, Ku SY, Kim SH, Choi YM, Kim JG, Moon SY

  • KMID: 2235683
  • Korean J Fertil Steril.
  • 2004 Dec;31(4):261-272.
OBJECTIVE: This study was performed to evaluate the possibility of prolonged culture of human embryonic stem cells (hESC; SNUhES2) on human amniotic fluid cells (hAFC), which had been storaged after...
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Induced Differentiation of Embryonic Stem Cells to Insulin Secreting Cells

Sung JH, Lim CK, Choi HW, Lee HS, Shin H, Jun JH, Yoon HS, Koong MK

  • KMID: 2235677
  • Korean J Fertil Steril.
  • 2004 Dec;31(4):209-216.
OBJECTIVE: Embryonic stem (ES) cells could be differentiated into the specific cell types by alternation of culture condition and modification of gene expression. This study was performed to evaluate the...
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Characterization of MACS Isolated Cells from Differentiated Human ES Cells

Cho JW, Lim CK, Shin MR, Bang KH, Koong MK, Jun JH

  • KMID: 2085180
  • Korean J Fertil Steril.
  • 2006 Sep;33(3):171-178.
OBJECTIVE: Human embryonic stem (ES) cells have a great potential in regenerative medicine and tissue engineering. The human ES cells could be differentiated into specific cell types by treatments of...
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In Vitro Neural Cell Differentiation Derived from Human Embryonic Stem Cells: Effects of PDGF-bb and BDNF on the Generation of Functional Neurons

Cho HJ, Kim EY, Lee YJ, Choi KH, Ahn SY, Park SP, Lim JH

  • KMID: 1864600
  • Korean J Fertil Steril.
  • 2002 Jun;29(2):117-127.
OBJECTIVE: This study was to investigate the generation of the functional neuron derived from human embryonic stem(hES,MB03) cells on in vitro neural cell differentiation system. METHODS: For neural progenitor cell formation...
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Directing Human Embryonic Stem Cells towards Functional Endothelial Cells Easily and without Purification

Gil CH, Ki BS, Seo J, Choi JJ, Kim H, Kim IG, Jung AR, Lee WY, Choi Y, Park K, Moon SH, Chung HM

Hemangioblasts or blood islands only arise in early development thereby the sources to obtain these bi-potential cells are limited. While previous studies have isolated both lineages in vitro through the...
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In vitro Neural Cell Differentiation of Genetically Modified Human Embryonic Stem Cells Expressing Tyrosine Hydroxylase

Shin HA, Kim EY, Lee KS, Cho HY, Kim YS, Lee WD, Park S, Lim JH

  • KMID: 1891202
  • Korean J Fertil Steril.
  • 2004 Mar;31(1):67-74.
OBJECTIVE: This study was to examine in vitro neural cell differentiation pattern of the genetically modified human embryonic stem cells expressing tyrosine hydroxylase (TH). MATERIALS AND METHODS: Human embryonic stem (hES,...
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Subretinal transplantation of putative retinal pigment epithelial cells derived from human embryonic stem cells in rat retinal degeneration model

Park UC, Cho MS, Park JH, Kim SJ, Ku SY, Choi YM, Moon SY, Yu HG

OBJECTIVE: To differentiate the human embryonic stem cells (hESCs) into the retinal pigment epithelium (RPE) in the defined culture condition and determine its therapeutic potential for the treatment of retinal...
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Establishment of Human Embryonic Stem Cells using Mouse Embryonic Fibroblasts and Human Fetal Fibroblasts as Feeder Cells

Cho HW, Ko KR, Kim MK, Lee JI, Sin SI, Lee DH, Kim KH, Lee KS

  • KMID: 2085171
  • Korean J Fertil Steril.
  • 2005 Jun;32(2):133-147.
OBJECTIVES: This study was carried out to establish human embryonic stem cells derived from frozen-thawed embryos using mouse embryonic fibroblasts (mEFs), human fetal skin and muscle fibroblasts as feeder cells,...
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The effects of BMP4 and BMP2 on Expression of Trophoblast-Specific Genes in Human Embryonic Stem Cells using SNU-hES3 cell line

Lee YU, Lee YK, Lee JE, Jang YK, Hwang JH

  • KMID: 2272734
  • Korean J Obstet Gynecol.
  • 2005 Nov;48(11):2618-2626.
OBJECTIVE: Human embryonic stem cell derived from blastocyst randomly differentiates into multiple cell types during embryoid body development. Bone morphogenetic proteins (BMPs) are members of the transforming growth factor type...
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Effects of Neurotrophic Factors on the Generation of Functional Dopamine Secretory Neurons Derived from in vitro Differentiated Human Embryonic Stem Cells

Lee KS, Kim EY, Shin HA, Cho HY, Wang KC, Kim YS, Lee HT, Chung KS, Lee WD, Park S, Lim JH

  • KMID: 1891197
  • Korean J Fertil Steril.
  • 2004 Mar;31(1):19-27.
OBJECTIVE: This study was to examine the in vitro neural cell differentiation patterns of human embryonic stem (hES) cells following treatment of various neurotrophic factors [basic fibroblast growth factor (bFGF),...
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