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Purification and Characterization of Intracellular Cellulase from Aspergillus oryzae ITCC-4857.01

Begum MF, Absar N

  • KMID: 2408782
  • Mycobiology.
  • 2009 Jun;37(2):121-127.
Purification and characterization of intracellular cellulase produced by A. oryzae ITCC-4857.01 are reported. The enzyme was purified by ion-exchange chromatography using DEAE-cellulose followed by Gel filtration. The purification achieved was...
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Studies on the protein biosynthesis in isolated periodontal ligament

Chung HI

  • KMID: 2271422
  • Korean J Orthod.
  • 1979 Dec;9(1):9-14.
The purpose of this study was to pursue the biosynthesis of proteins of human and bovine periodontal ligaments in vitro system. The excised periodontal ligaments from human and bovine were...
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Purification of heat-labile enterotoxin from an enterotoxin from an enterotoxigenic Escherichia coli of human origin by monoclonal immunoaffinity chromatography

Cho MJ, Chang WH, Choi MS, Kim IS, Kang JS, Park KH, Kim HK, Cha CY, Chung HK, Rhee KH

Heat-labile enterotoxin (LT) was purified from an enterotoxigenic Escherichia coli 015H11 of human origin. The purification steps included French pressure cell disruption of the bacteria, salting-out, DEAE-Sephacel on chromatography. Application...
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Xanthine Oxidase Stimulates the Enzyme Inactivation by Metal-Catalyzed Oxidation Systems

Chay KO

  • KMID: 2172399
  • Chonnam Med J.
  • 2001 Dec;37(4):357-365.
A cytosolic protein stimulating the inactivation of glutamine synthetase (GS) by metal-catalyzed oxidation (MCO) systems was purified and characterized from the rat lung. The protein was purified through the following...
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Purification of a New Antioxidnat Protein from the Hypoxia-Exposed Rat Lung

Jung YD, Chay KO, Song DU, Song H, Yang SY, Lee MW, Ahn BW

  • KMID: 2172519
  • Chonnam Med J.
  • 1998 Jun;34(1):87-95.
Hyperoxia enhances antioxidant potential in rat lung tissues. In this study, an antioxidant protein with Mr of -68 kDa has been purified to nearly homogeneity from the rat lung after...
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Purification of thrombospondin receptor (CD36) from human platelet membrane

Kim JH, Suk KH, Park NY, Kim SH

  • KMID: 2128623
  • Exp Mol Med.
  • 1997 Mar;29(1):31-34.
Thrombospondin receptor (CD36) has been recently identified in platelets and various cell lines as the receptor for thrombospondin, an adhesive protein required for irreversible aggregation of platelets as well as...
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Production and Characterization of a New alpha-Glucosidase Inhibitory Peptide from Aspergillus oryzae N159-1

Kang MG, Yi SH, Lee JS

An alpha-glucosidase inhibitor was developed from Aspergillus oryzae N159-1, which was screened from traditional fermented Korean foods. The intracellular concentration of the inhibitor reached its highest level when the fungus...
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Purification and Characterization of an Extracellular Alkaline Protease from Aspergillus niger C-15

Kim JD

An alkaline protease produced by Aspergillus niger C-15 was purified and characterized. The enzyme was purified 19.41-fold with a specific activity of 74150 U/mg and a recovery of 34.4% by...
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Purification of the major allergens from Korean Dermatophagoides pteronyssinus and production of the recombinant antigens

Kim KS, Park SW, Park JW, Hong CS, Oh SH

  • KMID: 2286081
  • J Asthma Allergy Clin Immunol.
  • 1999 Feb;19(1):91-102.
BACKGROUND: Purified major allergens of house dust mite are essential for evaluation of the allergic mechanism in molecular basis and development of new modalities of immunemodulation. OBJECTIVE: In this study, we...
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Purification and Characterisation of Extracellular Proteinase from Trichophyton rubrum

Kim DH, Lee YS, Yoo JI, Choi YH, Joo HY, Kim BS, Kim KS, Kim JA

  • KMID: 2080346
  • Korean J Med Mycol.
  • 1997 Jun;2(1):51-58.
BACKGROUND: Trichophyton rubrum is the most common dermatophyte isolated from human and has ability to invade the tissues such as stratum comeum, nail and hair. The potential role of proteinases...
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Immunolocalization of the 150 kDa protein in cyst fluid of Taenia solium metacestodes

Yang HJ, Chung YB

The 150 kDa protein of cyst fluid (CF) of Taenia solium metacestodes was purified by ammonium sulfate fractionation and Superose 6 HR gel filtration chromatography. The purified protein consisted of...
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Proteoglycan in porcine aortic tissue after cryopreservation

Shon YH

This study was to investigate the effects of cryopreservation on proteoglycans of arterial conduit tissue. Proteoglycans from fresh and cryopreserved porcine aorta tissues were extracted with 4 M guanidine...
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New Developments in the Immunological Understanding and of Serodiagnosis in Syphilis

Muller F

There are many serologic tests for syphilis. By means of the usual serologic tests, it is not possible to differentiate between patients who need therapy and those who are cured....
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Characterization of an Anti-gout Xanthine Oxidase Inhibitor from Pleurotus ostreatus

Jang IT, Hyun SH, Shin JW, Lee YH, Ji JH, Lee JS

We selected Pleurotus ostreatus from among several edible mushrooms because it has high anti-gout xanthine oxidase (XOD) inhibitory activity. The maximal amount of XOD inhibitor was extracted when the Pleurotus...
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Purification and Characterization of Transforming growth factor - beta1 from Human Platelets

Ko EJ, Lee JW, Nham SU, Choi EY, Chun GT, Yie SW, Kim PH

  • KMID: 2083008
  • Korean J Immunol.
  • 1998 Mar;20(1):1-8.
Transforming growth factor-j31 (TGF-p1) has potential for therapeutic use in common clinical conditions for which there are no adequate pharmacological agents. However, in vivo studies using TGF-p1 were hindered by...
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The Study of the Preparation of Purified Fibronectin from the Human Plasma

Kim JC, Kim IS, Kim HC, Shyn KH, Koo BS, Lee HS

  • KMID: 1949238
  • J Korean Ophthalmol Soc.
  • 1989 Aug;30(4):189-197.
Fibronectin(FN), a glycoprotein present in plasma and extracellular matrix, has been reported to be effective on various corneal disorders such as persistent epithelial defect, corneal trophic ulcer, herpetic keratitis, etc....
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Purification and Characterization of a Keratinase from a Feather-Degrading Fungus, Aspergillus flavus Strain K-03

Kim JD

  • KMID: 2312534
  • Mycobiology.
  • 2007 Dec;35(4):219-225.
A keratinolytic enzyme secreted by Aspergillus flavus K-03 cultured in feather meal basal medium (FMBM) containing 2% (w/v) chicken feather was purified and characterized. Keratinolytic enzyme secretion was the maximal...
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Analysis of the Chemical Constituents of Agaricus brasiliensis

Cho SM, Jang KY, Park HJ, Park JS

This study examined the chemical composition of A. blasiliensis and the chemical structural properties of an immuno-stimulating polysaccharide. The amino acids, free sugars, and organic acids by HPLC and fatty...
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Partial characterization of a 29 kDa cysteine protease purified from Taenia solium metacestodes

Kim JY, Yang HJ, Kim KS, Chung YB

A 29 kDa cysteine protease of Taenia solium metacestodes was purified by Mono Q anion-exchanger and Superose 6 HR gel filtration chromatography. The enzyme was effectively inhibited by cysteine protease...
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Partial Purification and Properties of a Cysteine Protease from Citrus Red Mite Panonychus citri

Hong SC, Her KH, Kim HU, Lee J, Lee SP, Chung YB

Several studies have reported that the citrus red mites Panonychus citri were an important allergen of citrus-cultivating farmers in Jeju Island. The aim of the present study was to purify...
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