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Evaluation of DNA Extraction Methods and Their Clinical Application for Direct Detection of Causative Bacteria in Continuous Ambulatory Peritoneal Dialysis Culture Fluids from Patients with Peritonitis by Using Broad-Range PCR

Kim SH, Jeong HS, Kim YH, Song SA, Lee JY, Oh SH, Kim HR, Lee JN, Kho WG, Shin JH

BACKGROUND: The aims of this study were to compare several DNA extraction methods and 16S rDNA primers and to evaluate the clinical utility of broad-range PCR in continuous ambulatory peritoneal...
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Performance of Kiestra Total Laboratory Automation Combined with MS in Clinical Microbiology Practice

Mutters NT, Hodiamont , de Jong MD, Overmeijer HPJ, van den Boogaard M, Visser CE

BACKGROUND: Microbiological laboratories seek technologically innovative solutions to cope with large numbers of samples and limited personnel and financial resources. One platform that has recently become available is the Kiestra...
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Detection of Lawsonia intracellularis in diagnostic specimens by one-step PCR

Suh DK, Lym SK, Bae YC, Lee KW, Choi WP, Song JC

  • KMID: 1103472
  • J Vet Sci.
  • 2000 Jun;1(1):33-37.
Lawsonia intracellularis is not culturable with a standard bacteriologic culture. One step PCR assay as a clinical diagnostic method was developed for the rapid detection of porcine proliferative enteritis (PPE)...
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A multiplex quantitative real-time polymerase chain reaction panel for detecting neurologic pathogens in dogs with meningoencephalitis

Han JI, Chang DW, Na KJ

Meningoencephalitis (ME) is a common inflammatory disorder of the central nervous system in dogs. Clinically, ME has both infectious and non-infectious causes. In the present study, a multiplex quantitative real-time...
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